One Conversation, One More Generation

Great News for Patients – Ovarian Tissue Cryopreservation
Now Considered Standard Care!

The American Society for Reproductive Medicine (ASRM) recently stated that “ovarian tissue banking is an acceptable fertility-preservation technique and is no longer considered experimental.” (Read ASRM article here.) ReproTech has always been a strong advocate for making all fertility preservation options available to patients and we have worked closely with some of the first hospitals offering ovarian tissue cryopreservation to pre- and post-pubertal females, beginning in 2010, through the Oncofertility Consortium. For some girls, freezing ovarian tissue before starting fertility threatening treatments due to cancer or other illnesses is the only method of preserving their fertility as they are too young to retrieve mature eggs. Later in life, when these girls and young women are ready to start a family, if they have trouble conceiving naturally, the hope is that their tissue retrieved before chemotherapy, or other treatments, can be transplanted back into their bodies allowing them to achieve pregnancy. Changing ovarian tissue cryopreservation from experimental to standard care is a huge step forward in being able to offer this procedure to more patients in more hospitals throughout the country and also helps to push for better insurance coverage for medical expenses related to preservation. A wonderful development for patients who are survivors of childhood cancer giving them the hope of future fertility.

ReproTech Participates in Experiment to Study Cryostorage Tank Performance

Published: October 24, 2019 - ReproTech, Ltd. CEO, Brent Hazelrigg, contributed to this experiment and article in the Journal of Assisted Reproduction & Genetics that provided useful information about cryostorage tank performance after an induced vacuum failure.

Assisted Reproduction Technologies

Click here for the link to the abstract.

Cryostorage tank failures: temperature and volume loss over time after induced failure by removal of insulative vacuum

Authors: Kimball O. Pomeroy, Michael L. Reed, Brian LoManto, Stanley G. Harris, W. Brent Hazelrigg, Dawn A. Kelk


To determine liquid nitrogen evaporation rates of intact liquid nitrogen storage tanks and tanks with their vacuum removed.

Donated storage tank performance (LN2 evaporation) was evaluated before and after induced vacuum failure. Vacuum of each tank was removed by drilling through the vacuum port. Temperature probes were placed 2 in. below the bottom of the styrofoam cap/plug, and tanks were weighed every 3 h. Evaporation rate and time from failure to the critical temperature was determined.

Storage tanks with failed vacuum have a much higher evaporation rate than those with intact vacuum; evaporation rates increased dramatically within 3 to 6 h in the smaller tanks, and time to complete depletion varied according to starting LN2 volume. Tanks with storage racks/specimens may have altered evaporation profiles compared to tanks without. Locating temperature probes 2 in. below the styrofoam cap/plug suggests that for most applications, alarms would sound approximately 1 h prior to reaching the critical warming temperature, approximately − 130 °C. External signs of vacuum loss were dramatic: vapor, frost, and audible movement of air.

For the first time, we have data on how liquid nitrogen storage tanks behave when their vacuum is removed. These findings are conservative; each lab must consider starting volume, tank size/capacity, function (storage or shipping), age, and pre-existing evaporation behavior in order to develop an emergency response to critical tank failure. Times to complete failure/evaporation and critical warming temperature after vacuum loss are different; these data should be considered when evaluating tank alarm systems.


A special thanks to those that donated tanks to this study: The World Egg Bank, Colorado State University’s Animal Reproduction Laboratory, ReproTech, Ltd, Yale Fertility Center, and Kaiser Permanente Center for Reproductive Health.

Authors’ contribution
All authors contributed to the study conception and design and contributed to material preparation and data collection. All authors contributed to data analysis, with final review by KOP. All authors contributed to and had opportunity to comment on the original version of the manuscript.

Compliance with ethical standards
Conflict of interest
There were no conflicts of interest reported by the authors, and no compensation was received by the authors for participation in this project. No commercial, State or Federal funding was utilized in this study; materials, monitoring devices, and tanks were available in-house or were donated for the purposes described in this manuscript.

Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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